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Normal blood film a zone of central pallor about one

third the size of the RBC diameter Platelet (small and

Blue-staining) An RBC is about two thirds the size of a normal lymphocyte Normal mature

lymphocyte minimal variation in size (anisocytosis) and shape (poikilocytosis)

This case is an autoimmune hemolytic anemia Spherocytes that lack central pallor

Reticulocyte large and blue Hereditary spherocytosis

Heinz body inclusions in glucose-6-phosphate dehydrogenase (G6PD) deficiency

methylene blue stain Sickle cell anemia

Megaloblastic anemia macro-ovalocytes The mean corpuscular volume can be mildly

increased in individuals recovering from blood loss or hemolytic anemia because the newly released RBCs, the reticulocytes,

are increased in size over normal RBCs, which decrease in size slightly with aging

The increased size of the RBCs (macrocytosis) is hard to appreciate in a blood smear. Compare the RBCs with the lymphocyte at the lower left

center This is a case of pernicious anemia

Hypochromic anemia, microscopic There is also increased anisocytosis

(variation in size) and poikilocytosis (variation in shape) This peripheral blood smear shows

a nucleated RBC in the center that contains basophilic stippling of the cytoplasm. This suggests a toxic

injury to the bone marrow, such as lead poisoning. Such stippling may also appear with severe anemia, such as a

megaloblastic anemia. Microangiopathic hemolytic anemia

myelofibrosis AMLacute promyelocytic leukemia Auer rods

AML Thank you

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